Total Internal Reflection Fluorescence Microscopy (TIRFM)

Revolution® TIRF

Total Internal Reflection Fluorescence Microscopy (TIRFM) provides an unrivalled technique for the study of single molecules and membrane related cell function, including adhesion, motility, endo- and exo-cytosis.

What is TIRF

In TIRFM the electromagnetic field of the TIR light extends into the sample beyond the interface by 100-200nm into the medium of lower refractive index. Since the field decreases exponentially along the z-axis, only a very thin section of the specimen undergoes fluorescence excitation.

TIRFM produces extremely high contrast, low background images, ideal for detection with our iXon3 EMCCD cameras and is a valuable tool in the study of e.g. cellular membrane traffic, cytoskeleton dynamics and single molecule studies.

Andor Revolution components provide:
  • Matched illuminator and detector for maximum performance
  • Flexible fiber-coupled 400-650 nm excitation compatible with all TIRF platforms
  • Multiple fiber outputs for confocal, TIRF and Photobleach
  • Integral AOTF – microsecond wavelength, shuttering and intensity control
  • Active blanking - precise synchronization and > 80 dB isolation

Components For TIRF